Hotspot

petA-psbJ

LSC · 65465-66489 · 1025 bp

Back to Wallichia

Pi 0.0226

MarkerSeek score 72.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1025
variable_sites: 23
pi: 0.0226
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.0	58.3	1177-1179 bp	1.0	79.2
petA-psbJ_p2	ATCCATTACGTGTCCAAGGC	CATATTCCGGGTTGGGTTCA	50.0	50.0	58.0	57.6	1173-1175 bp	1.0	78.3
petA-psbJ_p3	ATCCATTACGTGTCCAAGGC	GTTCATATTCCGGGTTGGGT	50.0	50.0	58.0	57.6	1176-1178 bp	1.0	78.3
petA-psbJ_p4	CATTACGTGTCCAAGGCCTT	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.2	58.3	1174-1176 bp	1.0	78.1
petA-psbJ_p5	CATTACGTGTCCAAGGCCTT	CATATTCCGGGTTGGGTTCA	50.0	50.0	58.2	57.6	1170-1172 bp	1.0	77.2