Hotspot

rbcL-accD

LSC · 59168-59971 · 804 bp

Back to Wallichia

Pi 0.00128

MarkerSeek score 62.10

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 804
variable_sites: 1
pi: 0.00128
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCAAATGGAGCCCTGAACTA	GGGTTCTTCACTTCCACTGG	50.0	55.0	57.9	58.1	994-1013 bp	1.0	80.0
rbcL-accD_p2	TAGCAAATGGAGCCCTGAAC	GGGTTCTTCACTTCCACTGG	50.0	55.0	57.9	58.1	996-1015 bp	1.0	80.0
rbcL-accD_p3	TGAAGCTAGCAAATGGAGCC	GGGTTCTTCACTTCCACTGG	50.0	55.0	58.2	58.1	1002-1021 bp	1.0	79.0
rbcL-accD_p4	CCGTGAAGCTAGCAAATGGA	GGGTTCTTCACTTCCACTGG	50.0	55.0	58.3	58.1	1005-1024 bp	1.0	78.9
rbcL-accD_p5	TCCGTGAAGCTAGCAAATGG	GGGTTCTTCACTTCCACTGG	50.0	55.0	58.3	58.1	1006-1025 bp	1.0	78.9