Hotspot

ndhC-trnV-UAC

LSC · 51854-53952 · 2099 bp

Back to Wallichia

Pi 0.00348

MarkerSeek score 63.50

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 2099
variable_sites: 7
pi: 0.00348
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCCTTCACTAACCGGTGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.1	57.8	2179-2243 bp	1.0	81.2
ndhC-trnV-UAC_p2	TAACCGGTGCTAAAACTCCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	2171-2235 bp	1.0	81.1
ndhC-trnV-UAC_p3	TCCTTCACTAACCGGTGCTA	AGCCCAATGCAACAAAATGC	50.0	45.0	58.1	58.5	2261-2325 bp	1.0	78.9
ndhC-trnV-UAC_p4	TAACCGGTGCTAAAACTCCG	AGCCCAATGCAACAAAATGC	50.0	45.0	57.9	58.5	2253-2317 bp	1.0	78.8
ndhC-trnV-UAC_p5	TCCTTCACTAACCGGTGCTA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.1	57.5	2178-2242 bp	1.0	78.5