Hotspot

rrn23

IRa · 129906-132715 · 2810 bp

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Pi 0
MarkerSeek score 23.70
Primer available yes
Label rrn23

Feature Metadata

label_name
rrn23
feature_type
rRNA
region
IRa
length_bp
2810
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
23.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn23_p1 AAACTTCTGTCGCTCCATCC GCACCTTAGGATGGCATAGC 50.0 55.0 57.9 58.5 2960 bp 1.0 75.5
rrn23_p2 GCCGTGACCTTCTCTTGAAT GCACCTTAGGATGGCATAGC 50.0 55.0 57.9 58.5 2985 bp 1.0 75.5
rrn23_p3 AAACTTCTGTCGCTCCATCC GCATCCATTTAGCAGCACCT 50.0 50.0 57.9 58.6 2974 bp 1.0 74.4
rrn23_p4 GCCGTGACCTTCTCTTGAAT GCATCCATTTAGCAGCACCT 50.0 50.0 57.9 58.6 2999 bp 1.0 74.4
rrn23_p5 GCTCTGGGGAAGTCGGAATA CGGCGGTTCGATCTATGATT 55.0 50.0 58.9 57.9 2953 bp 1.0 72.5