Hotspot

rps16

LSC · 4824-5955 · 1132 bp

Back to Vuhuangia

Pi 0.000884

MarkerSeek score 59.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1132
variable_sites: 1
pi: 0.000884
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	ATATACAAGCCGCTACCCCC	ACCGGTTCCGAATTAGAGGA	55.0	50.0	59.0	58.1	1189-1190 bp	1.0	70.8
rps16_p2	ATATACAAGCCGCTACCCCC	CAATTCCATACCGGTTCCGA	55.0	50.0	59.0	57.7	1198-1199 bp	1.0	69.4
rps16_p3	ATATACAAGCCGCTACCCCC	TCAATTCCATACCGGTTCCG	55.0	50.0	59.0	57.7	1199-1200 bp	1.0	69.4
rps16_p4	TATACAAGCCGCTACCCCC	ACCGGTTCCGAATTAGAGGA	57.9	50.0	58.6	58.1	1188-1189 bp	1.0	66.5
rps16_p5	TATATACAAGCCGCTACCCC	ACCGGTTCCGAATTAGAGGA	50.0	50.0	56.3	58.1	1190-1191 bp	1.0	65.2