Hotspot

ndhF

SSC · 109399-111630 · 2232 bp

Back to Vuhuangia

Pi 0.00134

MarkerSeek score 52.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 3
pi: 0.00134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGACCCGTTTCTGAAGAGTC	CCTTTCAAAAGAAGTAAGGTCCG	50.0	43.5	56.9	57.6	2356 bp	1.0	44.3
ndhF_p2	GACCCGTTTCTGAAGAGTCT	CCTTTCAAAAGAAGTAAGGTCCG	50.0	43.5	56.9	57.6	2355 bp	1.0	44.3
ndhF_p3	GACCCGTTTCTGAAGAGTCTT	CCTTTCAAAAGAAGTAAGGTCCG	47.6	43.5	57.6	57.6	2355 bp	1.0	42.1
ndhF_p4	AGACCCGTTTCTGAAGAGTCT	CCTTTCAAAAGAAGTAAGGTCCG	47.6	43.5	58.4	57.6	2356 bp	1.0	42.0
ndhF_p5	ACCCGTTTCTGAAGAGTCTT	CCTTTCAAAAGAAGTAAGGTCCG	45.0	43.5	56.4	57.6	2354 bp	1.0	40.5