Hotspot

atpF

LSC · 11774-13032 · 1259 bp

Back to Vuhuangia

Pi 0.000794

MarkerSeek score 59.20

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1259
variable_sites: 1
pi: 0.000794
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CGTCGGCTCGAATAGTTACC	GGGGCAAGGCGATAGAAAAA	55.0	50.0	58.2	58.5	1438 bp	1.0	73.8
atpF_p2	CGTCGGCTCGAATAGTTACC	GGGCAAGGCGATAGAAAAAG	55.0	50.0	58.2	57.1	1437 bp	1.0	71.3
atpF_p3	TTTCGTCGGCTCGAATAGTT	GGGGCAAGGCGATAGAAAAA	45.0	50.0	57.4	58.5	1441 bp	1.0	70.7
atpF_p4	TCGTCGGCTCGAATAGTTAC	GGGGCAAGGCGATAGAAAAA	50.0	50.0	57.3	58.5	1439 bp	1.0	69.9
atpF_p5	GTCGGCTCGAATAGTTACCA	GGGGCAAGGCGATAGAAAAA	50.0	50.0	57.1	58.5	1437 bp	1.0	69.0