Hotspot

trnV-GAC-rps12

IRa · 136948-138539 · 1592 bp

Back to Vuhuangia

Pi 0.000635
MarkerSeek score 60.00
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
IRa
length_bp
1592
variable_sites
1
pi
0.000635
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 GATGACTTCCACCACGTCAA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1668-1685 bp 1.0 77.5
trnV-GAC-rps12_p2 AACTGATGACTTCCACCACG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1672-1689 bp 1.0 77.4
trnV-GAC-rps12_p3 ATGACTTCCACCACGTCAAG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1667-1684 bp 1.0 77.4
trnV-GAC-rps12_p4 CTGATGACTTCCACCACGTC TGGCAACTTTACGTAAGGCA 55.0 45.0 58.3 57.7 1670-1687 bp 1.0 76.5
trnV-GAC-rps12_p5 GTCAAGGTGACACTCTACCG TGGCAACTTTACGTAAGGCA 55.0 45.0 57.7 57.7 1653-1670 bp 1.0 76.0