Hotspot

rpl20-rps12

LSC · 68862-69675 · 814 bp

Back to Vuhuangia

Pi 0.00123
MarkerSeek score 59.50
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
814
variable_sites
1
pi
0.00123
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 ACCCTCGAAAACTTGATGCA CGCCGAGGAACATGTACTAG 45.0 55.0 57.7 57.9 932 bp 1.0 77.0
rpl20-rps12_p2 CGGGCTATATATCCGCGTTT CGCCGAGGAACATGTACTAG 50.0 55.0 57.7 57.9 885 bp 1.0 76.8
rpl20-rps12_p3 ACCCTCGAAAACTTGATGCA ACTAGGGTGTATGTGCGACT 45.0 50.0 57.7 58.2 917 bp 1.0 76.7
rpl20-rps12_p4 CGGGCTATATATCCGCGTTT ACTAGGGTGTATGTGCGACT 50.0 50.0 57.7 58.2 870 bp 1.0 76.6
rpl20-rps12_p5 ACCCTCGAAAACTTGATGCA AATACAAGACAGCCCGTTCG 45.0 50.0 57.7 58.3 992 bp 1.0 75.7