Hotspot

ndhC-trnV-UAC

LSC · 50326-51279 · 954 bp

Back to Vuhuangia

Pi 0.00105

MarkerSeek score 59.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 954
variable_sites: 1
pi: 0.00105
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CGGGGCTAAAATCCCAGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1086 bp	1.0	77.4
ndhC-trnV-UAC_p2	AATCGGGGCTAAAATCCCAG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.6	57.8	1089 bp	1.0	75.6
ndhC-trnV-UAC_p3	CTCCGGCCCTTTTCTAATCG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.4	57.8	1104 bp	1.0	75.2
ndhC-trnV-UAC_p4	CGGGGCTAAAATCCCAGAAA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1085 bp	1.0	74.6
ndhC-trnV-UAC_p5	CGGGGCTAAAATCCCAGAAA	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	1074 bp	1.0	73.5