Hotspot

trnT-UGU-trnL-UAA

LSC · 46294-46983 · 690 bp

Back to Vuhuangia

Pi 0.00145

MarkerSeek score 59.60

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 690
variable_sites: 1
pi: 0.00145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	751 bp	1.0	77.1
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	783 bp	1.0	76.2
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	745 bp	1.0	75.8
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	55.0	50.0	57.9	57.5	783 bp	1.0	75.0
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	50.0	50.0	57.8	57.6	777 bp	1.0	74.9