Hotspot

psaA-ycf3

LSC · 41358-42003 · 646 bp

Back to Vuhuangia

Pi 0.00155

MarkerSeek score 59.90

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 646
variable_sites: 1
pi: 0.00155
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	TTGGTTGAAGATCACGGGAC	50.0	50.0	57.9	57.8	755-757 bp	1.0	78.0
psaA-ycf3_p2	GAAAAATGACCCGGTTTGGC	TTGGTTGAAGATCACGGGAC	50.0	50.0	58.2	57.8	773-775 bp	1.0	77.2
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TGGTTTGATCAAGCTGCTGA	50.0	45.0	57.9	57.7	829-831 bp	1.0	77.2
psaA-ycf3_p4	GAAAAATGACCCGGTTTGGC	TGGTTTGATCAAGCTGCTGA	50.0	45.0	58.2	57.7	847-849 bp	1.0	76.4
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	CGGAGGCTTGGTTTGATCAA	50.0	50.0	57.9	58.5	837-839 bp	1.0	75.6