Hotspot

petN-psbM

LSC · 28402-29423 · 1022 bp

Back to Vuhuangia

Pi 0.00196
MarkerSeek score 58.50
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1022
variable_sites
2
pi
0.00196
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 ACTCGTAGTGTGGGGAAGAA AGTTCCGACCGCTTTTCTAC 50.0 50.0 58.0 57.9 1111 bp 1.0 79.5
petN-psbM_p2 ACTCGTAGTGTGGGGAAGAA GTTCCGACCGCTTTTCTACT 50.0 50.0 58.0 57.9 1110 bp 1.0 79.5
petN-psbM_p3 TCGTAGTGTGGGGAAGAAGT AGTTCCGACCGCTTTTCTAC 50.0 50.0 58.0 57.9 1109 bp 1.0 79.5
petN-psbM_p4 TCGTAGTGTGGGGAAGAAGT GTTCCGACCGCTTTTCTACT 50.0 50.0 58.0 57.9 1108 bp 1.0 79.5
petN-psbM_p5 CGTAGTGTGGGGAAGAAGTG GTTCCGACCGCTTTTCTACT 55.0 50.0 57.9 57.9 1107 bp 1.0 78.9