Hotspot

matK-trnK-UUU

LSC · 3561-4254 · 694 bp

Back to Vuhuangia

Pi 0.00289

MarkerSeek score 60.50

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 694
variable_sites: 2
pi: 0.00289
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	AGGAAGTCGTGTTGTTGAGA	TGGGGTTGCTAACTCAATGG	45.0	50.0	56.7	57.8	788-789 bp	1.0	68.4
matK-trnK-UUU_p2	AGGAAGTCGTGTTGTTGAGA	GGGGTTGCTAACTCAATGGT	45.0	50.0	56.7	57.8	787-788 bp	1.0	68.4
matK-trnK-UUU_p3	GCACGATTATGAGCAAACACA	TGGGGTTGCTAACTCAATGG	42.9	50.0	57.5	57.8	839-840 bp	1.0	66.8
matK-trnK-UUU_p4	GCACGATTATGAGCAAACACA	GGGGTTGCTAACTCAATGGT	42.9	50.0	57.5	57.8	838-839 bp	1.0	66.8
matK-trnK-UUU_p5	AGCACGATTATGAGCAAACAC	TGGGGTTGCTAACTCAATGG	42.9	50.0	57.3	57.8	840-841 bp	1.0	64.6