Hotspot

rpl32-trnL-UAG

SSC · 111604-111971 · 368 bp

Back to Vrydagzynea

Pi 0.0168
MarkerSeek score 57.60
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
368
variable_sites
6
pi
0.0168
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGAAAAAGCTTTTACTGCCGC TCCTAAGAGCAGCGTGTCTA 42.9 50.0 58.0 57.9 502-506 bp 1.0 73.4
rpl32-trnL-UAG_p2 GAAAAAGCTTTTACTGCCGCT TCCTAAGAGCAGCGTGTCTA 42.9 50.0 58.0 57.9 501-505 bp 1.0 73.4
rpl32-trnL-UAG_p3 AGAAAAAGCTTTTACTGCCGC AGCAGCGTGTCTACCAATTT 42.9 45.0 58.0 57.8 495-499 bp 1.0 73.0
rpl32-trnL-UAG_p4 GAAAAAGCTTTTACTGCCGCT AGCAGCGTGTCTACCAATTT 42.9 45.0 58.0 57.8 494-498 bp 1.0 73.0
rpl32-trnL-UAG_p5 GAAAAAGCTTTTACTGCCGCT CTAAGAGCAGCGTGTCTACC 42.9 55.0 58.0 57.8 499-503 bp 1.0 72.9