Hotspot

rrn16-trnI-GAU

IRb · 100998-101299 · 302 bp

Back to Vrydagzynea

Pi 0.0331

MarkerSeek score 71.60

Primer available yes

Label rrn16-trnI-GAU

Feature Metadata

label_name: rrn16-trnI-GAU
feature_type: igs
region: IRb
length_bp: 302
variable_sites: 10
pi: 0.0331
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-trnI-GAU_p1	CAAGGTAGCCGTACTGGAAG	GGCACAACGACGCAATTATC	55.0	50.0	57.7	58.2	396 bp	1.0	78.1
rrn16-trnI-GAU_p2	AGTGACTGGAGTGAAGTCGT	GGCACAACGACGCAATTATC	50.0	50.0	58.3	58.2	418 bp	1.0	77.9
rrn16-trnI-GAU_p3	AAGTCGTAACAAGGTAGCCG	GGCACAACGACGCAATTATC	50.0	50.0	57.6	58.2	405 bp	1.0	77.6
rrn16-trnI-GAU_p4	AACAAGGTAGCCGTACTGGA	GGCACAACGACGCAATTATC	50.0	50.0	58.4	58.2	398 bp	1.0	77.6
rrn16-trnI-GAU_p5	ACAAGGTAGCCGTACTGGAA	GGCACAACGACGCAATTATC	50.0	50.0	58.4	58.2	397 bp	1.0	77.6