Hotspot

rpl33-rps18

LSC · 66324-66492 · 169 bp

Back to Vrydagzynea

Pi 0.024

MarkerSeek score 67.40

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 169
variable_sites: 4
pi: 0.024
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	TCGACACAATACATCCAATCA	TAATCGATTCGATCCCCCGA	38.1	50.0	55.0	58.1	346-351 bp	1.0	49.1
rpl33-rps18_p2	TCGACACAATACATCCAATCA	GGGGTAAACGCCTACGAAAA	38.1	50.0	55.0	58.2	318-323 bp	1.0	48.3
rpl33-rps18_p3	TCGACACAATACATCCAATCAA	TAATCGATTCGATCCCCCGA	36.4	50.0	55.8	58.1	346-351 bp	1.0	47.3
rpl33-rps18_p4	TCAAAAGTATACGATTCATGGGGA	TAATCGATTCGATCCCCCGA	37.5	50.0	57.8	58.1	295-300 bp	1.0	47.0
rpl33-rps18_p5	AGAATCGACACAATACATCCA	TAATCGATTCGATCCCCCGA	38.1	50.0	54.8	58.1	350-355 bp	1.0	46.7