Hotspot

psbF-psbE

LSC · 63541-63550 · 10 bp

Back to Vrydagzynea

Pi 0.1
MarkerSeek score 79.00
Primer available yes
Label psbF-psbE

Feature Metadata

label_name
psbF-psbE
feature_type
igs
region
LSC
length_bp
10
variable_sites
1
pi
0.1
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbF-psbE_p1 ACAGTAGGTACAGCCAGTCC GAGAACCGACAAGGGATTCC 55.0 55.0 58.4 58.0 165 bp 1.0 76.7
psbF-psbE_p2 ACAGTAGGTACAGCCAGTCC ACAGAGAACCGACAAGGGAT 55.0 50.0 58.4 58.4 168 bp 1.0 74.1
psbF-psbE_p3 ACAGTAGGTACAGCCAGTCC GAACCGACAAGGGATTCCAT 55.0 50.0 58.4 57.6 163 bp 1.0 73.6
psbF-psbE_p4 ACAGTAGGTACAGCCAGTCC AGAACCGACAAGGGATTCCA 55.0 50.0 58.4 58.6 164 bp 1.0 71.8
psbF-psbE_p5 AGTAGGTACAGCCAGTCCG GAGAACCGACAAGGGATTCC 57.9 55.0 58.1 58.0 163 bp 1.0 71.4