Hotspot

trnS-UGA-psbZ

LSC · 34738-34968 · 231 bp

Back to Vrydagzynea

Pi 0.0346

MarkerSeek score 59.30

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 231
variable_sites: 8
pi: 0.0346
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	AGCAAATACAACGGGTACACT	55.0	42.9	58.0	57.6	335-342 bp	1.0	70.4
trnS-UGA-psbZ_p2	GCTATCAACCACTCAGCCAT	AGCAAATACAACGGGTACACT	50.0	42.9	57.7	57.6	340-347 bp	1.0	67.9
trnS-UGA-psbZ_p3	TCAACCACTCAGCCATCTCT	AGCAAATACAACGGGTACACT	50.0	42.9	58.4	57.6	336-343 bp	1.0	67.8
trnS-UGA-psbZ_p4	CAACCACTCAGCCATCTCTC	AGCAAATACAACGGGTACAC	55.0	45.0	58.0	56.1	335-342 bp	1.0	66.2
trnS-UGA-psbZ_p5	CAACCACTCAGCCATCTCTC	GCAAATACAACGGGTACACT	55.0	45.0	58.0	56.1	334-341 bp	1.0	66.2