Hotspot

trnT-GGU-psbD

LSC · 31075-32079 · 1005 bp

Back to Vrydagzynea

Pi 0.0139

MarkerSeek score 66.20

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1005
variable_sites: 14
pi: 0.0139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1165-1194 bp	1.0	79.5
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1168-1197 bp	1.0	79.4
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1155-1184 bp	1.0	78.5
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	CCCTCCGTAACCAATCATCC	55.0	55.0	58.1	57.7	1139-1168 bp	1.0	78.4
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1158-1187 bp	1.0	78.3