Hotspot

trnD-GUC-trnY-GUA

LSC · 30088-30370 · 283 bp

Back to Vrydagzynea

Pi 0.0424

MarkerSeek score 63.10

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 283
variable_sites: 12
pi: 0.0424
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	ATTCCAATTCGGGACTGACG	ATTCCTTCTTCCTGGGTCGA	50.0	50.0	58.0	58.0	463-465 bp	1.0	81.8
trnD-GUC-trnY-GUA_p2	ATTCCAATTCGGGACTGACG	TTCCTTCTTCCTGGGTCGAT	50.0	50.0	58.0	58.0	462-464 bp	1.0	81.8
trnD-GUC-trnY-GUA_p3	ATTCCAATTCGGGACTGACG	GAACCGATGACTTACGCCTT	50.0	50.0	58.0	58.0	1053-1055 bp	1.0	81.0
trnD-GUC-trnY-GUA_p4	ATTCCAATTCGGGACTGACG	CCATGGCGTTACTCTACCAC	50.0	55.0	58.0	58.1	1032-1034 bp	1.0	80.6
trnD-GUC-trnY-GUA_p5	AAAGAAAGGGGCGGGTTAAG	GAACCGATGACTTACGCCTT	50.0	50.0	58.1	58.0	1630 bp	0.5	59.3