Hotspot

psbK-psbI

LSC · 7256-7706 · 451 bp

Back to Vrydagzynea

Pi 0.02

MarkerSeek score 67.40

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 451
variable_sites: 9
pi: 0.02
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TTCTTCACGCCCAGGATTAC	50.0	50.0	57.9	57.6	589-601 bp	1.0	77.7
psbK-psbI_p2	TGGCAAGCTGCTGTAAGTTT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.3	57.6	586-598 bp	1.0	75.7
psbK-psbI_p3	TTGGCAAGCTGCTGTAAGTT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.3	57.6	587-599 bp	1.0	75.7
psbK-psbI_p4	TTTGGCAAGCTGCTGTAAGT	TTCTTCACGCCCAGGATTAC	45.0	50.0	58.3	57.6	588-600 bp	1.0	75.7
psbK-psbI_p5	GTTTGGCAAGCTGCTGTAAG	GGATTACGTCCCGGATCATT	50.0	50.0	57.9	57.2	576-588 bp	1.0	74.3