Hotspot

rps16-trnQ-UUG

LSC · 6105-6639 · 535 bp

Back to Vrydagzynea

Pi 0.0287

MarkerSeek score 67.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 535
variable_sites: 15
pi: 0.0287
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	ACAAAAGAAGAATGGGGCGT	45.0	45.0	58.1	58.0	657-669 bp	1.0	82.6
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	ACGGGTTTTGGTCCTGTTAC	45.0	50.0	58.1	58.0	620-632 bp	1.0	82.1
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	597-609 bp	1.0	81.9
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	601-613 bp	1.0	80.8
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	CGGGTTTTGGTCCTGTTACT	45.0	50.0	58.1	57.7	619-631 bp	1.0	80.2