Hotspot

trnK-UUU-rps16

LSC · 4350-4925 · 576 bp

Back to Vrydagzynea

Pi 0.033
MarkerSeek score 68.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
576
variable_sites
19
pi
0.033
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GCTCAACCCACAGGAACT 50.0 55.6 58.2 56.8 697-709 bp 1.0 55.0
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GCTCAACCCACAGGAACT 50.0 55.6 58.2 56.8 696-708 bp 1.0 55.0
trnK-UUU-rps16_p3 TCAGTCGCGGTCTTAGAAAC GCTCAACCCACAGGAACT 50.0 55.6 57.7 56.8 778-790 bp 1.0 53.4
trnK-UUU-rps16_p4 CAGTCGCGGTCTTAGAAACT GCTCAACCCACAGGAACT 50.0 55.6 57.7 56.8 777-789 bp 1.0 53.3
trnK-UUU-rps16_p5 TACCGTTGAGTTAGCAACCC GCTCAACCCACAGGAACT 50.0 55.6 57.5 56.8 683-695 bp 1.0 52.7