Hotspot

ycf1

SSC · 10728-16178 · 5451 bp

Back to Vriesea

Pi 0.00339

MarkerSeek score 40.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5451
variable_sites: 34
pi: 0.00339
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTTGTCTTTTGTGATTCGTTCT	AGGAAATTCAAAAAGTAAACCCA	34.8	30.4	56.7	54.9	237 bp	1.0	41.4
ycf1_p2	TCTTGTCTTTTGTGATTCGTTCT	AGGAAATTCAAAAAGTAAACCCAA	34.8	29.2	56.7	55.6	237 bp	1.0	41.4
ycf1_p3	TCTTGTCTTTTGTGATTCGTTCT	TGAAGGAAATTCAAAAAGTAAACCCA	34.8	30.8	56.7	58.1	240 bp	1.0	41.3
ycf1_p4	TCTTGTCTTTTGTGATTCGTTCT	GAAGGAAATTCAAAAAGTAAACCCA	34.8	32.0	56.7	56.7	239 bp	1.0	41.3
ycf1_p5	TCTTGTCTTTTGTGATTCGTTCT	TGAAGGAAATTCAAAAAGTAAACCC	34.8	32.0	56.7	56.7	240 bp	1.0	41.3