Hotspot

atpF

LSC · 53216-54611 · 1396 bp

Back to Vriesea

Pi 0.00143

MarkerSeek score 59.70

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1396
variable_sites: 3
pi: 0.00143
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACTAATTTCGTCGGCTCGAA	GGGCGAGCGAAGTAATAGAA	45.0	50.0	57.4	57.5	1569-1591 bp	1.0	71.0
atpF_p2	GGCTCGAAGGGTTACCATTA	GGGCGAGCGAAGTAATAGAA	50.0	50.0	57.0	57.5	1557-1579 bp	1.0	68.2
atpF_p3	ACTAATTTCGTCGGCTCGAA	AAGGGGCGAGCGAAGTAATA	45.0	50.0	57.4	58.9	1572-1594 bp	1.0	68.0
atpF_p4	GGCTCGAAGGGTTACCATTAG	GGGCGAGCGAAGTAATAGAA	52.4	50.0	58.2	57.5	1557-1579 bp	1.0	66.9
atpF_p5	ACTAATTTCGTCGGCTCGAA	GGGCGAGCGAAGTAATAGAAA	45.0	47.6	57.4	58.2	1569-1591 bp	1.0	65.6