Hotspot

rpl16-rps3

LSC · 126482-126626 · 145 bp

Back to Vriesea

Pi 0.00805

MarkerSeek score 59.50

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 145
variable_sites: 2
pi: 0.00805
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AGTTTATAGCGGGGGTCAGT	TCCCCTACAAACAATTCGCG	50.0	50.0	58.1	58.6	335-338 bp	1.0	76.0
rpl16-rps3_p2	AGCGGGGGTCAGTTTATTTT	TCCCCTACAAACAATTCGCG	45.0	50.0	57.4	58.6	328-331 bp	1.0	72.2
rpl16-rps3_p3	GTTTATAGCGGGGGTCAGTT	TCCCCTACAAACAATTCGCG	50.0	50.0	57.3	58.6	334-337 bp	1.0	71.4
rpl16-rps3_p4	AGTTTATAGCGGGGGTCAGT	CCCTACAAACAATTCGCGCT	50.0	50.0	58.1	59.2	333-336 bp	1.0	70.9
rpl16-rps3_p5	AGTTTATAGCGGGGGTCAGT	GTTCCCCTACAAACAATTCGC	50.0	47.6	58.1	57.8	337-340 bp	1.0	70.7