Hotspot

trnP-UGG-psaJ

LSC · 110223-110559 · 337 bp

Back to Vriesea

Pi 0.00148
MarkerSeek score 37.60
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
337
variable_sites
1
pi
0.00148
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
37.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC TAGACCCGCTAAAGACCCAA 50.0 50.0 58.1 58.1 497 bp 1.0 78.9
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA TAGACCCGCTAAAGACCCAA 50.0 50.0 58.1 58.1 496 bp 1.0 78.9
trnP-UGG-psaJ_p3 ACAGGATTTGAACCCGTGAC TAGACCCGCTAAAGACCCAA 50.0 50.0 58.1 58.1 508 bp 1.0 78.8
trnP-UGG-psaJ_p4 CAGGATTTGAACCCGTGACA TAGACCCGCTAAAGACCCAA 50.0 50.0 58.1 58.1 507 bp 1.0 78.8
trnP-UGG-psaJ_p5 TGACAGGATTTGAACCCGTG TAGACCCGCTAAAGACCCAA 50.0 50.0 58.1 58.1 510 bp 1.0 78.8