Hotspot

trnG-GCC-psbZ

LSC · 97109-97425 · 317 bp

Back to Vriesea

Pi 0.00631
MarkerSeek score 52.70
Primer available yes
Label trnG-GCC-psbZ

Feature Metadata

label_name
trnG-GCC-psbZ
feature_type
igs
region
LSC
length_bp
317
variable_sites
4
pi
0.00631
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-psbZ_p1 AATCGAACCCGCATCTTCTC TGCTTCTTCTGATGGTTGGC 50.0 50.0 58.1 58.5 484 bp 1.0 76.5
trnG-GCC-psbZ_p2 GAATCGAACCCGCATCTTCT TGCTTCTTCTGATGGTTGGC 50.0 50.0 58.1 58.5 485 bp 1.0 76.5
trnG-GCC-psbZ_p3 AATCGAACCCGCATCTTCTC TGGTTGGCCAAGTAACAAGA 50.0 45.0 58.1 57.3 472 bp 1.0 74.3
trnG-GCC-psbZ_p4 GAATCGAACCCGCATCTTCT TGGTTGGCCAAGTAACAAGA 50.0 45.0 58.1 57.3 473 bp 1.0 74.3
trnG-GCC-psbZ_p5 AATCGAACCCGCATCTTCTC TGGTTGGCCAAGTAACAAGAA 50.0 42.9 58.1 58.0 472 bp 1.0 72.1