Hotspot

ycf3-psaA

LSC · 91111-91743 · 633 bp

Back to Vriesea

Pi 0.00395

MarkerSeek score 62.60

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: LSC
length_bp: 633
variable_sites: 5
pi: 0.00395
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	GTTGAAAATCACGAAGCGCT	CCCATTCCTCGAAAGACGTT	45.0	50.0	57.7	57.9	735-747 bp	1.0	77.9
ycf3-psaA_p2	GCTATAGCGCTTACTCCAGG	CCCATTCCTCGAAAGACGTT	55.0	50.0	57.6	57.9	779-791 bp	1.0	77.5
ycf3-psaA_p3	CCGATCAAGCTGCTGAGTAT	CCCATTCCTCGAAAGACGTT	50.0	50.0	57.5	57.9	808-820 bp	1.0	76.1
ycf3-psaA_p4	TCCGATCAAGCTGCTGAGTA	CCCATTCCTCGAAAGACGTT	50.0	50.0	58.5	57.9	809-821 bp	1.0	76.1
ycf3-psaA_p5	TGAAAATCACGAAGCGCTTC	CCCATTCCTCGAAAGACGTT	45.0	50.0	57.4	57.9	733-745 bp	1.0	75.7