Hotspot

psbK-psbI

LSC · 48609-49028 · 420 bp

Back to Vriesea

Pi 0.00833

MarkerSeek score 48.80

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 420
variable_sites: 6
pi: 0.00833
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAAGCCGCTGTAAGTTTTCG	GGATTGCGTCCTGGATCATT	50.0	50.0	58.0	58.0	539-542 bp	1.0	80.3
psbK-psbI_p2	AGCCGCTGTAAGTTTTCGAT	GGATTGCGTCCTGGATCATT	45.0	50.0	57.9	58.0	537-540 bp	1.0	79.9
psbK-psbI_p3	AAGCCGCTGTAAGTTTTCGA	GGATTGCGTCCTGGATCATT	45.0	50.0	58.1	58.0	538-541 bp	1.0	79.7
psbK-psbI_p4	GCCGCTGTAAGTTTTCGATG	GGATTGCGTCCTGGATCATT	50.0	50.0	57.8	58.0	536-539 bp	1.0	79.4
psbK-psbI_p5	GCAAGCCGCTGTAAGTTTTC	GGATTGCGTCCTGGATCATT	50.0	50.0	58.3	58.0	540-543 bp	1.0	78.2