Hotspot

rpl21

SSC · 108937-109350 · 414 bp

Back to Vittaria

Pi 0.467

MarkerSeek score 78.10

Primer available yes

Label rpl21

Feature Metadata

label_name: rpl21
feature_type: gene
region: SSC
length_bp: 414
variable_sites: 172
pi: 0.467
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl21_p1	GCTAATAGCGGACATACGGG	AAACTTCTTGAACGTCCGCT	55.0	45.0	58.0	58.1	1019 bp	0.5	59.5
rpl21_p2	GCTAATAGCGGACATACGGG	AACTTCTTGAACGTCCGCTT	55.0	45.0	58.0	58.1	1018 bp	0.5	59.5
rpl21_p3	GCTAATAGCGGACATACGGG	TGCGGAATATAGCGCAGTTT	55.0	45.0	58.0	58.1	1417 bp	0.5	59.5
rpl21_p4	GATCCTAACATTGACGCGGT	AAACTTCTTGAACGTCCGCT	50.0	45.0	58.1	58.1	1379 bp	0.5	59.1
rpl21_p5	GATCCTAACATTGACGCGGT	AACTTCTTGAACGTCCGCTT	50.0	45.0	58.1	58.1	1378 bp	0.5	59.1