Hotspot

ndhG

SSC · 114481-115074 · 594 bp

Back to Vittaria

Pi 0.464

MarkerSeek score 81.70

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 594
variable_sites: 244
pi: 0.464
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TGTGCCATCGAGCCTATTTC	TGTCCCATCAATCTCCCAGT	50.0	50.0	58.0	58.0	1785 bp	0.5	59.4
ndhG_p2	TACTGAATGTGCCATCGAGC	TGTCCCATCAATCTCCCAGT	50.0	50.0	58.1	58.0	1792 bp	0.5	59.3
ndhG_p3	CCTGCGTGTTCCTTTTCTCT	TGTCCCATCAATCTCCCAGT	50.0	50.0	58.1	58.0	1626 bp	0.5	58.8
ndhG_p4	GCAGCTCCAACGGCTATTAT	TGTCCCATCAATCTCCCAGT	50.0	50.0	58.1	58.0	1379 bp	0.5	58.8
ndhG_p5	TCCTGCGTGTTCCTTTTCTC	TGTCCCATCAATCTCCCAGT	50.0	50.0	58.1	58.0	1627 bp	0.5	58.8