Hotspot

ndhF

SSC · 106490-108670 · 2181 bp

Back to Vittaria

Pi 0.45

MarkerSeek score 79.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2181
variable_sites: 833
pi: 0.45
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CGAATTGGAGCTTCGTGAGA	TACCCAGGGAGAACCAACAT	50.0	50.0	58.0	57.9	2749 bp	0.5	59.5
ndhF_p2	CTCCCAAGAGGCGTAGTAGA	TACCCAGGGAGAACCAACAT	55.0	50.0	57.9	57.9	2911 bp	0.5	59.1
ndhF_p3	GATCAACCTCACAATCCCCC	TACCCAGGGAGAACCAACAT	55.0	50.0	57.9	57.9	2979 bp	0.5	59.1
ndhF_p4	AATATGCAGGAGAGTCGGGA	TACCCAGGGAGAACCAACAT	50.0	50.0	57.9	57.9	2779 bp	0.5	58.9
ndhF_p5	CGAATTGGAGCTTCGTGAGA	TTACCCAGGGAGAACCAACA	50.0	50.0	58.0	58.2	2750 bp	0.5	58.5