Hotspot

ndhD

SSC · 111853-113364 · 1512 bp

Back to Vittaria

Pi 0.498

MarkerSeek score 81.00

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1512
variable_sites: 647
pi: 0.498
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CTTTTTGGAGTTGGGACCCA	TGAGCGTCCGTGTCTATTTG	50.0	50.0	57.9	58.0	2282 bp	0.5	59.3
ndhD_p2	TTGGAGTTGGGACCCAAAAG	TGAGCGTCCGTGTCTATTTG	50.0	50.0	57.9	58.0	2278 bp	0.5	59.3
ndhD_p3	CTTTTTGGAGTTGGGACCCA	TACCTCAGCTCATACCCGAG	50.0	55.0	57.9	58.0	2185 bp	0.5	59.2
ndhD_p4	TTGGAGTTGGGACCCAAAAG	TACCTCAGCTCATACCCGAG	50.0	55.0	57.9	58.0	2181 bp	0.5	59.2
ndhD_p5	TTGGAGTTGGGACCCAAAAG	CCCCTAGAACGGAAGATTGC	50.0	55.0	57.9	58.0	2341 bp	0.5	59.0