Hotspot

ndhA

SSC · 115889-117800 · 1912 bp

Back to Vittaria

Pi 0.454

MarkerSeek score 78.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 1912
variable_sites: 679
pi: 0.454
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CTCCAGATGAAGGTAGGGGT	ATGAGAGCGCTTTACCTTGG	55.0	50.0	57.8	58.0	2656 bp	0.5	58.3
ndhA_p2	CGAAATCGTTCACCTGGGAA	ATGAGAGCGCTTTACCTTGG	50.0	50.0	58.2	58.0	2363 bp	0.5	58.2
ndhA_p3	CTCCAGATGAAGGTAGGGGT	AATGGCAAGGAGGAGGAGAT	55.0	50.0	57.8	58.1	2941 bp	0.5	57.8
ndhA_p4	CTCCAGATGAAGGTAGGGGT	ATGGCAAGGAGGAGGAGATT	55.0	50.0	57.8	58.1	2940 bp	0.5	57.8
ndhA_p5	GTATGCGCCCTAATGAGGTT	ATGAGAGCGCTTTACCTTGG	50.0	50.0	57.7	58.0	2638 bp	0.5	57.7