Hotspot

chlN

SSC · 125072-126463 · 1392 bp

Back to Vittaria

Pi 0.482

MarkerSeek score 80.60

Primer available yes

Label chlN

Feature Metadata

label_name: chlN
feature_type: gene
region: SSC
length_bp: 1392
variable_sites: 565
pi: 0.482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN_p1	CTGTGAAGGACCTACTGGGA	GGTTATGGTGGCGTAGACTG	55.0	55.0	58.1	58.1	2634 bp	0.5	59.0
chlN_p2	TCTGTGAAGGACCTACTGGG	GGTTATGGTGGCGTAGACTG	55.0	55.0	58.1	58.1	2635 bp	0.5	59.0
chlN_p3	CTGTGAAGGACCTACTGGGA	CTGTGTAGAAGCTGGTGGAC	55.0	55.0	58.1	57.9	2617 bp	0.5	58.8
chlN_p4	CTGTGAAGGACCTACTGGGA	GACTGTGTAGAAGCTGGTGG	55.0	55.0	58.1	57.9	2619 bp	0.5	58.8
chlN_p5	TCTGTGAAGGACCTACTGGG	CTGTGTAGAAGCTGGTGGAC	55.0	55.0	58.1	57.9	2618 bp	0.5	58.8