Hotspot

ycf1-chlN

SSC · 124722-125071 · 350 bp

Back to Vittaria

Pi 0.455

MarkerSeek score 77.60

Primer available yes

Label ycf1-chlN

Feature Metadata

label_name: ycf1-chlN
feature_type: igs
region: SSC
length_bp: 350
variable_sites: 147
pi: 0.455
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-chlN_p1	AAGATATCGGAGCTCTGCCT	AGTTACTCGTCCATTGCGAC	50.0	50.0	58.0	58.0	1156 bp	0.5	59.9
ycf1-chlN_p2	AACACAGGATTTGGCCACAT	AGTTACTCGTCCATTGCGAC	45.0	50.0	58.0	58.0	914 bp	0.5	59.8
ycf1-chlN_p3	AAGATATCGGAGCTCTGCCT	ACGGCAACAACATTAATGCG	50.0	45.0	58.0	58.0	1707 bp	0.5	59.7
ycf1-chlN_p4	AAGATATCGGAGCTCTGCCT	CGGCAACAACATTAATGCGT	50.0	45.0	58.0	58.0	1706 bp	0.5	59.7
ycf1-chlN_p5	AAGATATCGGAGCTCTGCCT	TCAGGCTGGTTACCTTCTCA	50.0	50.0	58.0	58.0	1797 bp	0.5	59.7