Hotspot

trnR-ACG-rrn16

IRb · 84963-85899 · 937 bp

Back to Vittaria

Pi 0.0365

MarkerSeek score 78.00

Primer available yes

Label trnR-ACG-rrn16

Feature Metadata

label_name: trnR-ACG-rrn16
feature_type: igs
region: IRb
length_bp: 937
variable_sites: 31
pi: 0.0365
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-rrn16_p1	GTGTGGTACGAAACTCCGTT	CCAGGATCAAACTCTCCACG	50.0	55.0	58.1	58.0	978-1065 bp	1.0	85.2
trnR-ACG-rrn16_p2	AAGTGTGGTACGAAACTCCG	CCAGGATCAAACTCTCCACG	50.0	55.0	57.9	58.0	980-1067 bp	1.0	85.1
trnR-ACG-rrn16_p3	GTGTGGTACGAAACTCCGTT	CCACTGGAAACACCACTAGG	50.0	55.0	58.1	57.8	1052-1139 bp	1.0	83.6
trnR-ACG-rrn16_p4	AAGTGTGGTACGAAACTCCG	CCACTGGAAACACCACTAGG	50.0	55.0	57.9	57.8	1054-1141 bp	1.0	83.6
trnR-ACG-rrn16_p5	GTGTGGTACGAAACTCCGTT	GGAAACACCACTAGGATGCC	50.0	55.0	58.1	58.2	1047-1134 bp	1.0	83.1