Hotspot

ndhF

SSC · 115562-117811 · 2250 bp

Pi 0.00141

MarkerSeek score 31.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2250
variable_sites: 34
pi: 0.00141
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACTTCCGAAACGAAGGGAAC	GGTGAAGTTACTTGACCGCA	50.0	50.0	58.1	58.1	2968-2994 bp	1.0	80.6
ndhF_p2	ACTTCCGAAACGAAGGGAAC	ACTTGACCGCAGTAGATCCT	50.0	50.0	58.1	58.1	2959-2985 bp	1.0	80.5
ndhF_p3	GAACTTCCGAAACGAAGGGA	GGTGAAGTTACTTGACCGCA	50.0	50.0	57.8	58.1	2970-2996 bp	1.0	80.4
ndhF_p4	AGAACTTCCGAAACGAAGGG	GGTGAAGTTACTTGACCGCA	50.0	50.0	57.8	58.1	2971-2997 bp	1.0	80.3
ndhF_p5	GAACTTCCGAAACGAAGGGA	ACTTGACCGCAGTAGATCCT	50.0	50.0	57.8	58.1	2961-2987 bp	1.0	80.2