Hotspot

ndhD-psaC

SSC · 123308-123441 · 134 bp

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Pi 0.0118
MarkerSeek score 55.60
Primer available yes
Label ndhD-psaC

Feature Metadata

label_name
ndhD-psaC
feature_type
igs
region
SSC
length_bp
134
variable_sites
7
pi
0.0118
unique_haplotype_count
20
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-psaC_p1 TGCGGATATCGGCAAAACTA GCGTCGGTTGTAAGAGATGT 45.0 50.0 57.4 58.0 287-292 bp 1.0 79.8
ndhD-psaC_p2 TGCGGATATCGGCAAAACTA TGCGTCGGTTGTAAGAGATG 45.0 50.0 57.4 58.0 288-293 bp 1.0 79.8
ndhD-psaC_p3 TGCGGATATCGGCAAAACTA GTGAATCTGCCTGTCCAACA 45.0 50.0 57.4 58.1 269-274 bp 1.0 79.3
ndhD-psaC_p4 TGCGGATATCGGCAAAACTA TGTGAATCTGCCTGTCCAAC 45.0 50.0 57.4 58.1 270-275 bp 1.0 79.3
ndhD-psaC_p5 TGCGGATATCGGCAAAACTA TGAATCTGCCTGTCCAACAG 45.0 50.0 57.4 57.8 268-273 bp 1.0 78.7