Hotspot

ndhF-rpl32

SSC · 117812-119030 · 1219 bp

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Pi 0.00392
MarkerSeek score 50.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1219
variable_sites
44
pi
0.00392
unique_haplotype_count
28
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAGAAGTCCCACTCCTAGT ATTTCGCTAACGCAAAAGCC 55.0 45.0 57.5 58.0 1396-1412 bp 1.0 80.0
ndhF-rpl32_p2 GGAGAAGTCCCACTCCTAGT GCCATCCCTTCCTTTTCCAA 55.0 50.0 57.5 58.1 1363-1379 bp 1.0 79.7
ndhF-rpl32_p3 GGAGAAGTCCCACTCCTAGT CGCTAACGCAAAAGCCTTTA 55.0 45.0 57.5 57.4 1392-1408 bp 1.0 75.4
ndhF-rpl32_p4 GGAGAAGTCCCACTCCTAGT AACGCAAAAGCCTTTAACGC 55.0 45.0 57.5 58.5 1389-1404 bp 0.964912 74.1
ndhF-rpl32_p5 GGAGAAGTCCCACTCCTAGT CTCGCCATCCCTTCCTTTTC 55.0 55.0 57.5 58.6 1366-1382 bp 0.964912 72.7