Hotspot

ycf1-ndhF

IRb · 115437-115561 · 125 bp

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Pi 0.0555
MarkerSeek score 76.50
Primer available yes
Label ycf1-ndhF

Feature Metadata

label_name
ycf1-ndhF
feature_type
igs
region
IRb
length_bp
125
variable_sites
23
pi
0.0555
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1-ndhF_p1 TCGATGAAACGGAAGAGATCC GGTGGACGCATCTCCTCTTA 47.6 55.0 57.6 58.9 327-342 bp 1.0 69.1
ycf1-ndhF_p2 TCGATGAAACGGAAGAGATCC GTGGACGCATCTCCTCTTATC 47.6 52.4 57.6 58.0 326-341 bp 1.0 68.1
ycf1-ndhF_p3 TCGATGAAACGGAAGAGATCC TGGACGCATCTCCTCTTATCT 47.6 47.6 57.6 58.0 325-340 bp 1.0 67.9
ycf1-ndhF_p4 TCGATGAAACGGAAGAGATCC GTGGACGCATCTCCTCTTAT 47.6 50.0 57.6 56.8 326-341 bp 1.0 66.8
ycf1-ndhF_p5 TCGATGAAACGGAAGAGATCC CAAATATGTAGGGGGTGGACG 47.6 52.4 57.6 58.2 340-355 bp 1.0 66.7