Hotspot

rps19-rpl2

IRb · 89216-89284 · 69 bp

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Pi 0.0166
MarkerSeek score 58.70
Primer available yes
Label rps19-rpl2

Feature Metadata

label_name
rps19-rpl2
feature_type
igs
region
IRb
length_bp
69
variable_sites
6
pi
0.0166
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.97
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19-rpl2_p1 TTCCCACGTTGTGTTAAGCA CACAACCCCTTGGGGTTATC 45.0 55.0 58.0 58.2 243-246 bp 1.0 82.4
rps19-rpl2_p2 GATGTCCGGGACCAAGTTAC CACAACCCCTTGGGGTTATC 55.0 55.0 58.0 58.2 275-278 bp 1.0 82.1
rps19-rpl2_p3 AGATGTCCGGGACCAAGTTA CACAACCCCTTGGGGTTATC 50.0 55.0 58.1 58.2 276-279 bp 1.0 81.8
rps19-rpl2_p4 TAGATGTCCGGGACCAAGTT CACAACCCCTTGGGGTTATC 50.0 55.0 58.1 58.2 277-280 bp 1.0 81.8
rps19-rpl2_p5 GATGTCCGGGACCAAGTTAC TTGGGGTTATCCTGCACTTG 55.0 50.0 58.0 57.8 266-269 bp 1.0 81.7