Hotspot

petD-rpoA

LSC · 82583-82798 · 216 bp

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Pi 0.0137
MarkerSeek score 53.00
Primer available yes
Label petD-rpoA

Feature Metadata

label_name
petD-rpoA
feature_type
igs
region
LSC
length_bp
216
variable_sites
10
pi
0.0137
unique_haplotype_count
17
primer_available
yes
alignment_reliability
0.98
markerseek_score
53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD-rpoA_p1 GGTTGGGTATTGGAGCAACA AGATGTAAAACAGTTATTGGGCA 50.0 34.8 58.1 56.4 319-328 bp 1.0 47.4
petD-rpoA_p2 TGGTTGGGTATTGGAGCAAC AGATGTAAAACAGTTATTGGGCA 50.0 34.8 58.1 56.4 320-329 bp 1.0 47.4
petD-rpoA_p3 GGTTGGGTATTGGAGCAACA TGGGCATTCTAGAAAAACATTTCA 50.0 33.3 58.1 57.3 302-311 bp 1.0 47.0
petD-rpoA_p4 TGGTTGGGTATTGGAGCAAC TGGGCATTCTAGAAAAACATTTCA 50.0 33.3 58.1 57.3 303-312 bp 1.0 47.0
petD-rpoA_p5 GGTTGGGTATTGGAGCAACA ACAGTTATTGGGCATTCTAGA 50.0 38.1 58.1 54.3 310-319 bp 1.0 46.7