Hotspot

ndhC-trnV-UAC

LSC · 54514-55640 · 1127 bp

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Pi 0.00232
MarkerSeek score 48.60
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1127
variable_sites
18
pi
0.00232
unique_haplotype_count
18
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGGTCCTTTACTAATCGGAGC AGAAGGTCTACGGTTCGAGT 47.6 50.0 57.2 57.8 1259-1283 bp 1.0 66.5
ndhC-trnV-UAC_p2 GGTCCTTTACTAATCGGAGCT AGAAGGTCTACGGTTCGAGT 47.6 50.0 56.9 57.8 1258-1282 bp 1.0 64.2
ndhC-trnV-UAC_p3 TGGTCCTTTACTAATCGGAGC GAAGGTCTACGGTTCGAGTC 47.6 55.0 57.2 57.5 1258-1282 bp 1.0 63.7
ndhC-trnV-UAC_p4 CTGGTCCTTTACTAATCGGAGC AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.3 57.8 1260-1284 bp 1.0 62.7
ndhC-trnV-UAC_p5 TGGTCCTTTACTAATCGGAGC GTTCGAGTCCGTATAGCCCT 47.6 55.0 57.2 58.7 1247-1271 bp 1.0 62.6