Hotspot

psbZ-trnG-GCC

LSC · 39427-40117 · 691 bp

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Pi 0.00924
MarkerSeek score 62.10
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
691
variable_sites
50
pi
0.00924
unique_haplotype_count
42
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TTGCTTCTCCTGATGGTTGG AATCGAACCCGCATCTTCTC 50.0 50.0 57.8 58.1 795-863 bp 1.0 86.6
psbZ-trnG-GCC_p2 TTGCTTCTCCTGATGGTTGG GAATCGAACCCGCATCTTCT 50.0 50.0 57.8 58.1 796-864 bp 1.0 86.6
psbZ-trnG-GCC_p3 GCTTCTCCTGATGGTTGGTC AATCGAACCCGCATCTTCTC 55.0 50.0 58.3 58.1 793-861 bp 1.0 86.2
psbZ-trnG-GCC_p4 GCTTCTCCTGATGGTTGGTC GAATCGAACCCGCATCTTCT 55.0 50.0 58.3 58.1 794-862 bp 1.0 86.2
psbZ-trnG-GCC_p5 TTGCTTCTCCTGATGGTTGG GCATTTCTTTACCTTGGGCG 50.0 50.0 57.8 57.7 824-892 bp 1.0 84.7