Hotspot

trnC-GCA-petN

LSC · 29724-30570 · 847 bp

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Pi 0.00298
MarkerSeek score 54.00
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
847
variable_sites
30
pi
0.00298
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTTGTATCGTTTTGGCGGC AGCCCAAGCGAGACTTACTA 45.0 50.0 57.9 58.2 915-981 bp 1.0 84.8
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 859-925 bp 1.0 84.8
trnC-GCA-petN_p3 TTTTGTATCGTTTTGGCGGC AGAGTCCACTTCTTCCCCAT 45.0 50.0 57.9 57.6 976-1042 bp 1.0 83.1
trnC-GCA-petN_p4 TTTCCCCAGTTCAAATCCGG AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.1 57.6 920-986 bp 1.0 83.0
trnC-GCA-petN_p5 GGGGGACTGCAAATCCTTTT AGCCCAAGCGAGACTTACTA 50.0 50.0 58.4 58.2 877-943 bp 1.0 82.5