Hotspot

atpF-atpH

LSC · 14638-15137 · 500 bp

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Pi 0.00424
MarkerSeek score 51.20
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
500
variable_sites
14
pi
0.00424
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 CAGTGACCCAAGGAAACGAA CGGAGGGAAAAATACGAGGT 50.0 50.0 58.0 57.3 638-662 bp 1.0 79.2
atpF-atpH_p2 CAGTGACCCAAGGAAACGAA CGAGGCGGAGGGAAAAATAC 50.0 55.0 58.0 58.7 643-667 bp 1.0 79.1
atpF-atpH_p3 CAGTGACCCAAGGAAACGAA GAGGCGGAGGGAAAAATACG 50.0 55.0 58.0 58.7 642-666 bp 1.0 79.1
atpF-atpH_p4 CAGTGACCCAAGGAAACGAA GCGGAGGGAAAAATACGAGG 50.0 55.0 58.0 58.7 639-663 bp 1.0 79.1
atpF-atpH_p5 CAGTGACCCAAGGAAACGAA GGCGGAGGGAAAAATACGAG 50.0 55.0 58.0 58.7 640-664 bp 1.0 79.1