Hotspot

trnK-UUU-rps16

LSC · 4294-5160 · 867 bp

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Pi 0.00425
MarkerSeek score 47.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
867
variable_sites
31
pi
0.00425
unique_haplotype_count
36
primer_available
yes
alignment_reliability
0.99
markerseek_score
47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 ACAAGGTTTTGTGCCGATCT TCTATCCCAATGAGCCGTCT 45.0 50.0 58.0 57.9 1307-1376 bp 1.0 84.3
trnK-UUU-rps16_p2 ACAAGGTTTTGTGCCGATCT GCCGTACGAGGAGAAAACTT 45.0 50.0 58.0 57.9 1367-1436 bp 1.0 84.2
trnK-UUU-rps16_p3 ACAAGGTTTTGTGCCGATCT AGCCGTCTATCGAATCGTTG 45.0 50.0 58.0 57.9 1295-1364 bp 1.0 83.9
trnK-UUU-rps16_p4 CTCATGGGAGAAGCCTTTCC GCCGTACGAGGAGAAAACTT 55.0 50.0 58.0 57.9 1564-1633 bp 1.0 83.4
trnK-UUU-rps16_p5 CTCATGGGAGAAGCCTTTCC TCTATCCCAATGAGCCGTCT 55.0 50.0 58.0 57.9 1504-1573 bp 1.0 83.4